This study was designed to evaluate the effects of antimicrobial agents on the platelet
aggregation.
Fifty microliters of the various concentrations of ampicillin sodium (1,2,3, and 5 ㎍/㎖),
chloramphenicol(4,10,15, and 20 ㎍/㎖), and rifamycine SV(1,2,3, and 5 ㎍/㎖) were
incubated with 450 ㎕ of the platelet rich plasma (PRP) from the healthy volunteers for
30 minutes at room temperature, Then the platelet aggregation studies with aggregating
agents (ADP, collagen and ristocetin) were performed by turbidimetric method.
The results were as follows.
1. The ADP- and the collagen-induced platelet aggregations were inhibited by
ampicillin sodium in dose-related pattern and by the high concentrations of
chloramphenicol. Four micrograms per milliliters of chloramphenicol did not affect the
ADP-and collagen-induced platelet aggregations. Rifamycine SV inhibited the ADP-and
the collagen-induced platelet aggregations, but its effect was not dose-related as in
ampicillin sodium.
2. Ristocetin-induced platelet aggregations were almost completely inhibited by the
antimicrobials used in this study.
From these results, inhibition of platelet aggregation by the antimicrobials appeals to
occur at the platelet membrane including receptors for the aggregating agents. In other
words, the antimicrobials interfere with the binding of aggregating agents to the
receptors.
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